Identifies a cell surface protein of unknown function selectively expressed in gastrointestinal stromal tumors (GIST) and complements other markers such as c-kit (CD117). It identifies the vast majority of both c-kit negative and Platelet-derived Growth Factor Receptor Alpha mutated GIST cases that may still benefit from imatinib mesylate, an inhibitor of the Kit tyrosine kinase. DOG1 immunoreactivity is seen in fewer cases of mesanchymal, epithelial tumors and melanomas when compared with c-kit and will increase the accuracy of GIST diagnosis.
A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen typeorOne (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody orderedBlock and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.