An intenstinal-specific transcription factor that regulates both the proliferation and differentiation of intestinal epithelial cells. It is expressed in the nuclei of epithelial cells throughout the intestine, from duodenum to rectum, and is highly expressed in colorectal adenocarcinomas and at lower levels in the vast majority of non-colorectal GI carcinomas (those primary to the pancreatobiliary tract and stomach). It is also expressed in neuroendocrine carcinomas of the GI tract, but not in the normal gastric mucosa. It is useful in identifying metastatic carcinioma of colonic or other GI tract origin when presented with an unknown primary tumor.
A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen typeorOne (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody orderedBlock and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.