A multimeric cell surface-associated protein expressed in T cells in association with the T cell antigen receptor. It begins to be expressed during T cell development in the thymus and is the most specific T-cell antibody. It is aberrantly decreased in a subset of T cell neoplasms and expressed in normal thymocytes, peripheral T-cells, NK cells, and Purkinje cells of the cerebellum. NK cells express the epsilon chain of CD3 in the cytoplasm and in diseased cells, CD3 stains most T-cell lymphomas and rarely B-cell lymphomas.
A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen typeorOne (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody orderedBlock and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.