Identifies villin, an actin binding protein found in human renal epithelial cells and is a marker of carcinomas of GI tract origin. Villin, along with CDX2, is a highly specific and fairly sensitive marker of colorectal as well as noncolorectal GI tract (pancreatobiliary tract) derived carcinomas. In the latter tumors, a brush border pattern is characteristic. A subset of lung adenocarcinomas are also villin positive.
A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen typeorOne (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody orderedBlock and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.