Identifies a transmembrane protein that is a specific differentiation product of urothelial cellsand is a specific marker of transitional cell epithelium and tumors. In non-neoplastic urothelium, UPIII is expressed in the luminal surface plasmalemma of superficial cells. It detects half of urothelial carcinomas, whereas many non-urothelial carcinomas are negative. Because UP expression was found to be absent after malignant transformation in normal urothelium and bladder cancer specimens, UP expression might reflect the malignant potential of urothelial cancer cells as well as being cytodifferential markers of urothelial cells. While highly specific, UPIII is a marker of low sensitivity for urothelial carcinomas.
A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen typeorOne (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody orderedBlock and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.