All Tests

Standard Leukemia/Lymphoma Panel – 27 markers

ClearLLab 10C Standard Leukemia/Lymphoma Panel – 27 markers; Available as global and tech-only. Markers are CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD11b, CD13, CD14, CD15, CD16, CD19, CD20, CD33, CD34, CD38, CD45, CD56, CD64, CD117, CD123, CD200, HLA-DR, Kappa, Lambda, TCR-γδ.

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CLINICAL SIGNIFICANCE: Useful to aid in diagnosis of leukemia and lymphoma, and for post-treatment follow-up.

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Flow cytometry, also known as flow immunophenotyping or fluorescence activated cell count (FACS) is a technology that can rapidly count, sort, and analyze different cell types by creating a fluid suspension of cells with labeled targets to pass through an electronic detection system. This technique is widely used in diagnostics, biomarker detection, evaluating gene expression, and measuring both new and total synthesized DNA, allowing for simultaneous analysis of multiple physical and biochemical properties with one sample.

Our 10-color flow cytometry technique provides a precise characterization of various cell populations, improves detection of low-level abnormalities, and increases success with smaller specimens, delivering more specific and meaningful results for you and your patients.

For the standard leukemia / lymphoma panel we use the ClearLLab 10C system which is an integrated FDA cleared and CE-marked IVD leukemia and lymphoma (L&L) immunophenotyping solution for lymphoid and myeloid lineages using the dry DURA Innovations technology. ClearLLab Control Cells are the first application specific control cells for L&L immunophenotyping.

Our hematopathologist and Chief Medical Officer, Dr. Adrian Padurean Palmer, was one of the four principal investigators instrumental in Beckman Coulter’s ClearLLab 10C system application and FDA approval. *

*Hedley BD, Cheng G, Keeney M, Kern W,Padurean A, Luider J, Chin-Yee I, Lowes LE, Rohrbach J, Ortega R, Smit A, Lo K, Magari R, Tejidor L. A Multi-center Study Evaluation of the ClearLLab 10C Panels. Cytometry B Clin Cytom. 2021 Mar;100(2):225-234. (https://pubmed.ncbi.nlm.nih.gov/32667744/ )

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Synonyms
Flow
Turnaround Time
Global: 24-48 hrs, Tech-Only: 24 hrs
Methodology
Flow Cytometry
Specimen Requirements
  • Bone Marrow Aspirate: 1-2 mL EDTA. Sodium heparin is acceptable. Lithium heparin or ACD (pale yellow/no gel separator) is not acceptable. Please provide recent CBC report.
  • Peripheral Blood: 1-2 mL EDTA. Sodium heparin is acceptable. Lithium heparin or ACD (pale yellow/no gel separator) is not acceptable. Please provide recent CBC report.
  • Fresh Bone Marrow Core Biopsy: 1-2cm core (length) tissue in RPMI
  • Fresh/Unfixed Tissue: 0.2 cm3 minimum in RPMI
  • Fluids and FNAs: Equal parts RPMI and specimen volume
  • CSF: 1-2 mL recommended
  • Note: Please exclude biopsy needles, blades, and other foreign objects from transport tubes. These can compromise specimen viability and yield, and create hazards for employees.
    • Storage and Transportation

    Specimens should be received at Vitro Molecular within 72 hours from collection to assure sample integrity and acceptable cell viability. Refrigerate specimen. Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen.

    CPT Codes
    88184(x1), 88185(x26), 88189(x1)