Identifies a 100kDa glycoprotein present in high concentration in the prostate gland and its secretions. It is specific to the benign or malignant epithelial cells of the prostate gland and stains prostatic stroma, urethra, and basal cells negatively. PSAP activity is lost in epithelial cells injured due to inflammation, infarction, etc. and in areas of squamous metaplasia of the prostatic acini show loss of PSAP activity. Nearly all metastases of prostatic carcinomas, irrespective of site, demonstrate PSAP activity.
A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen typeorOne (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody orderedBlock and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.