Identifies a protein found in cytoplasmic granules of cytotoxic T-lymphocytes (CTLs), which bind to cells that express foreign antigens and induce them to lyse. Perforin expression is significantly induced in CD8 positive T cells, but to a lesser extent in gamma/delta T cells and NK cells, and may be useful in detecting perforin in CTLs in severe cases of graft versus host disease, chronic renal rejection, and peripheral T cell lymphomas. It also may be useful for the detection of NK cell lymphomas.
A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen typeorOne (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody orderedBlock and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.