Identifies a paternally imprinted protein which is an inhibitor of several G1 cyclin complexes and is a negative regulator of cell proliferation. Expression of this protein is lost in cytotrophoblasts of molar pregnancy. The gene encoding human p57 is located on chromosome 11p15.5, a region implicated in both sporadic cancers, Wilm's tumor, and Beckwith Wiedemann syndrome, making it a tumor suppressor candidate. The combination of analysis of p57 expression and ploidy (e.g. by FISH) can help define complete moles, incomplete moles, and hydropic normal villi. It is also useful in differentiating between complete hydatidiform mole (no nuclear p57 expression) and partial hydatidiform mole or spontaneous abortion (normal expression).
A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen typeorOne (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody orderedBlock and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.