A nuclear protein that is expressed in the germ cells of the testis and ovary. NUT midline carcinomas (NMCs) are aggressive tumors with non-diagnostic morphology that overlaps with many other poorly differentiated tumors but is defined by the presence of chromosomal rearrangement involving the NUT gene on chromosome 15q14, which causes protein overexpression. NUT overexpression by IHC may also be seen in other diseases, such as porocarcinoma, so a diagnosis of NUT midline carcinoma should not be based on NUT IHC positivity alone.
A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen typeorOne (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody orderedBlock and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.