Detects a mismatch repair protein involved in maintaining the integrity of genetic information. Mutations in the MLH1 gene have been reported to be found in tumors with microsatellite instability (MSI). Loss of gene expression can help define MSI type colonic carcinomas, a subset of sporadic carcinomas, and breast cancer. Loss of expression of MLH1 has also been reported in acute lymphoblastic leukemia, endometrial carcinoma, gastric carcinoma, and ovarian carcinoma.
A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen typeorOne (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody orderedBlock and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.