Identifies tyrosinase, a copper-containing metalloglycoprotein that is the rate limiting enzyme for controlling the production of melanin. It is a marker of melanocytic cells and tumors and is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this antibody showed tyrosinase in melanotic as well as amelanotic variants. It is a less sensitive marker of melanoma than HMB-45 and MART-1.
A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen typeorOne (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody orderedBlock and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.