Identifies lymphoid enhancer-binding factor 1, a nuclear transcription factor in the WNT/beta-catenin signaling pathway that is normally expressed in T cells and immature pro-B cells, but not in normal mature B cells. LEF1 overexpression is highly associated with CLL/SLL among small B-cell lymphomas and may serve as a useful marker for diagnosis and differential diagnosis of the disease, but it is almost always negative in mantle cell lymphoma, marginal zone lymphoma, and low-grade follicular lymphoma, and therefore may be helpful in distinguishing CLL/SLL from mantle cell lymphoma.
A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen typeorOne (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody orderedBlock and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.