The target of this test is the lambda light chain immunoglobulin mRNA in the cytoplasm of immunoblastic cells, plasma cells, and plasmacytoid cells. The detection of mRNA transcripts can be a useful surrogate for protein expression to determine the light chain restriction status for plasma cell and plasmacytoid B cell populations and aids in the distinction between neoplastic and reactive lymphoid proliferations and the evaluation of multiple myeloma, plasmacytoma, lymphomas with plasmacytoid features, immunoblastic lymphomas, and reactive plasma cell proliferations.
A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen typeorOne (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody orderedBlock and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.