This antibody reacts with proteins found in most ductal, glandular, and transitional epithelium of the urinary tract and bile duct epithelial cells. Lung and breast epithelium stain positive, and colon and prostate epithelial cells are negative. It also reacts with many benign and malignant epithelial lesions, e.g. adenocarcinomas of the ovary, breast, and lung. Transitional cell carcinomas are positive and most prostate cancers are negative. Co-analysis with expression of CK20 can be helpful in the identification of the primary site of carcinomas presenting at metastatic sites.
A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen typeorOne (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody orderedBlock and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.