HMB-45 (SP-100)

Identifies the gp100 protein, a melanoma-specific antigen found in premelanosomes. The antibody reacts with melanoma cells, nevus cells, and neonatal melanocytes, and because of this, it is a highly sensitive and specific marker of melanoma. It is expressed on the majority of malignant melanoma cases as well as on tumors of melanocytic differentiation, and it is also an excellent marker of clear cell sarcoma as well as PEComas.

Synonyms
Turnaround Time
24 hrs
Methodology
IHC
Specimen Requirements

A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen typeorOne (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody orderedBlock and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.

Storage and Transportation

Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.

CPT Codes
88342 x 1 or 88341 x 1