Identifies a highly conserved actin-bundly protein expressed predominantly in dendritic cells. Because lymphoid cells, myeloid cells, and plasma cells are negative for staining and Reed-Sternberg cells in Hodgkin lymphoma are positive, fascin expression can be used to distinguish between anaplastic large cell lymphoma (negative) and Hodgkin lymphoma (positive). Epstein-Barr virus may induce expression of fascin in B-cells.
A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen typeorOne (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody orderedBlock and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.