A cytoplasmic antigen expressed at all stages of B cell maturation, from lymphoblasts through plasma cells. It first appears at the pre B-cell stage and persits until the plasma cell stage where it is found as an intracellular component. It is found in the majority of acute leukemias of precursor B-cell type, B-cell lines, B-cell lymphomas, and in some myelomas. Expression is decreased in most cases of classical Hodgkin lymphoma and in a subset of non-Hodgkin B cell lymphomas, and is not present in myeloid cells or T-cells.
A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen typeorOne (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody orderedBlock and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.