CD14 is a glycoprotein expressed on mature monocytes, histiocytes/macrophages, Langerhans cells, neutrophils to a lesser degree, and facilitates phagocytic uptake. It stains normal macrophages/monocytes, granulocytes, Langerhans cells, dendritic cells, and B cells. It also distinguishes neoplasms of monocytic and Langerhans cell origin from other hematolymphoid neoplasms. Positive staining in diseased cells indicates B-cell chronic lymphocytic leukemmia, follicular center cell lymphoma, diffuse large B cell lymphoma, and acute myeloid leukemia.
A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred specimen typeorOne (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (all cut at 4-5 microns) for each test/antibody orderedBlock and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Use cold pack for transport. Make sure cold pack is not in direct contact with specimen.